Article
NMR docking of a substrate into the X-ray structure of the Asp-21-->Glu mutant of staphylococcal nuclease.
Biochemistry - 5 Jul 1994
Weber D J, Libson A M, Gittis A G, Lebowitz M S, Mildvan A S
Abstract excerpt
To understand the structural basis of the 1500-fold decrease in catalytic activity of the D21E mutant of staphylococcal nuclease in which an aspartate ligand of the essential Ca2+ has been enlarged to glutamate, the conformation of the enzyme-bound substrate dTdA has been determined by NMR methods and has been docked into the X-ray structure of the D21E mutant (Libson, A. M., Gittis, A.G., & Lattman, E. E....
Topics
- Cobalt
- Computer Simulation
- Crystallography, X-Ray
- Electron Spin Resonance Spectroscopy
- Magnetic Resonance Spectroscopy
- Manganese
- Metals
- Micrococcal Nuclease
- Models, Molecular
- Mutation
- Phosphorus