Article
Crystal structures of the binary Ca2+ and pdTp complexes and the ternary complex of the Asp21-->Glu mutant of staphylococcal nuclease. Implications for catalysis and ligand binding.
Biochemistry - 5 Jul 1994
Libson A M, Gittis A G, Lattman E E
Abstract excerpt
The crystal structure of the Asp21-->Glu mutant (D21E) of staphylococcal nuclease (SNase) has been determined in three different complex forms. The structure of the D21E ternary complex in which D21E is bound to both Ca2+ and the transition-state analogue, thymidine 3',5'-diphosphate (pdTp), was...
Topics
- Binding Sites
- Calcium
- Catalysis
- Crystallography, X-Ray
- DNA Mutational Analysis
- Ligands
- Micrococcal Nuclease
- Models, Molecular
- Mutation
- Structure-Activity Relationship
- Thymine Nucleotides