Article
Global analysis of the acid-induced and urea-induced unfolding of staphylococcal nuclease and two of its variants.
Biochemistry - 4 Feb 1997
Ionescu R M, Eftink M R
Abstract excerpt
We have studied the equilibrium unfolding staphylococcal nuclease and two of its variants, V66W and V66W', over two perturbation axes (acid-induced unfolding as a function of urea concentration and urea-induced unfolding as a function of pH). The transitions were monitored by simultaneous measure...
Topics
- Circular Dichroism
- Crystallography, X-Ray
- Genetic Variation
- Hydrogen-Ion Concentration
- Kinetics
- Micrococcal Nuclease
- Models, Structural
- Mutagenesis, Site-Directed
- Point Mutation
- Protein Conformation
- Protein Denaturation
- Protein Folding
- Protein Structure, Secondary
- Recombinant Proteins
- Thermodynamics
- Urea