Article
Deletion of the omega-loop in the active site of staphylococcal nuclease. 2. Effects on protein structure and dynamics.
Biochemistry - 16 Apr 1991
Baldisseri D M, Torchia D A, Poole L B, Gerlt J A
Abstract excerpt
It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition, both E43 delta SNase and D43 delta SNase are significantly more stable than their respective...
Topics
- Binding Sites
- Escherichia coli
- Magnetic Resonance Spectroscopy
- Micrococcal Nuclease
- Mutation
- Protein Conformation
- Structure-Activity Relationship
- X-Ray Diffraction