Article
Molecular cloning and expression in Escherichia coli of a gene coding for bovine S100A1 protein and its Glu32-->Gln and Glu73-->Gln mutants.
Acta biochimica Polonica - 1 Jan 1997
Bolewska K, Kozłowska H, Goch G, Mikołajek B, Bierzyński A
Abstract excerpt
Calcium binding S100A1 protein consists of two S100 alpha subunits. On the basis of sequence homology to other S100 proteins it is believed that the binding loops are formed by amino-acid residues 19-32 and 62-73 of S100 alpha polypeptide chain. In the oxidized form of the protein the subunits ar...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Calcium-Binding Proteins
- Cattle
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Escherichia coli
- Glutamic Acid
- Glutamine
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- S100 Proteins
- Spectrophotometry, Ultraviolet