Article
Cloning and expression in Escherichia coli of the structural gene coding for the monomeric protein of the S layer of Thermus thermophilus HB8.
Journal of bacteriology - 1 Sept 1991
Faraldo M L, de Pedro M A, Berenguer J
Abstract excerpt
The gene coding for the 100 kDa monomeric protein (P100) of the S layer of Thermus thermophilus HB8 has been cloned in the Escherichia coli plasmid vector pUC9. Recombinant plasmids were selected by colony screening with anti-P100 rabbit antiserum. The gene, named slpA (for surface layer protein...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Blotting, Western
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Restriction Mapping
- Thermus