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A hemi-nested, allele specific, whole blood PCR assay for the detection of the factor V Leiden mutation.

Thrombosis and haemostasis - 1 Jun 1997

Sinclair G D, Low S, Poon M C

Abstract excerpt

We describe a novel hemi-nested, allele specific whole blood PCR assay for detection of the factor V Leiden mutation associated with the plasma defect, activated protein C resistance. This assay utilizes 5 microliters of whole blood without prior DNA extraction. The hemi-nested design, employing an outer primer pair in combination with nested, allele specific primers obviates the need for restriction enzyme...

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PMID: 9241749

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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