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Article

Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines.

FEBS letters - 8 Feb 1993

Hardy F, Vriend G, Veltman O R, van der Vinne B, Venema G, Eijsink V G

Abstract excerpt

The thermostability of neutral proteases has been shown to depend on autolysis which presumably occurs in flexible regions of the protein. In an attempt to rigidify such a region in the neutral protease of Bacillus stearothermophilus, residues in the solvent-exposed 63-69 loop were replaced by proline. The mutations caused large positive (Ser-65-->Pro, Ala-69-->Pro) or negative (Thr-63-->Pro, Tyr-66-->Pro)...

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Identifiers

PMID: 8428638
DOI: 10.1016/0014-5793(93)81497-n

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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