Article
Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines.
FEBS letters - 8 Feb 1993
Hardy F, Vriend G, Veltman O R, van der Vinne B, Venema G, Eijsink V G
Abstract excerpt
The thermostability of neutral proteases has been shown to depend on autolysis which presumably occurs in flexible regions of the protein. In an attempt to rigidify such a region in the neutral protease of Bacillus stearothermophilus, residues in the solvent-exposed 63-69 loop were replaced by proline. The mutations caused large positive (Ser-65-->Pro, Ala-69-->Pro) or negative (Thr-63-->Pro, Tyr-66-->Pro)...
Topics
- Bacterial Proteins
- Endopeptidases
- Enzyme Stability
- Geobacillus stearothermophilus
- Hot Temperature
- Mutation
- Proline
- Protein Conformation