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Article

Development and use of a conditional antisense mutagenesis system in mycobacteria.

FEMS microbiology letters - 1 Sept 1997

Parish T, Stoker N G

Abstract excerpt

Expression from a 2.3 kb region upstream of the inducible acetamidase gene from Mycobacterium smegmatis was shown to be upregulated by acetamide. A DNA fragment containing the start of the M. smegmatis hisD gene was cloned in front of the promoter, such that the antisense message was produced. When this construct was induced in vivo, the bacteria became phenotypically histidine auxotrophs; this auxotrophy was...

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PMID: 9297833
DOI: 10.1111/j.1574-6968.1997.tb12637.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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