Article
Development and use of a conditional antisense mutagenesis system in mycobacteria.
FEMS microbiology letters - 1 Sept 1997
Parish T, Stoker N G
Abstract excerpt
Expression from a 2.3 kb region upstream of the inducible acetamidase gene from Mycobacterium smegmatis was shown to be upregulated by acetamide. A DNA fragment containing the start of the M. smegmatis hisD gene was cloned in front of the promoter, such that the antisense message was produced. Wh...
Topics
- Acetamides
- Amidohydrolases
- Antisense Elements (Genetics)
- DNA Primers
- DNA, Bacterial
- Gene Expression Regulation, Bacterial
- Genetic Vectors
- Histidine
- Molecular Sequence Data
- Mutagenesis
- Mycobacterium
- Phenotype