Article
Cloning of mycobacterial histidine synthesis genes by complementation of a Mycobacterium smegmatis auxotroph.
Molecular microbiology - 1 Oct 1992
Hinshelwood S, Stoker N G
Abstract excerpt
Histidine-requiring auxotrophs of Mycobacterium smegmatis were isolated following N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of these mutants, his5, was transformed with an M. smegmatis shuttle cosmid library, and complementing clones were isolated at a frequency of approximately 1%. A 2.3 kb fragment was subcloned and sequenced, and found to contain the start of an operon including the hisD gene and...
Topics
- ATP Phosphoribosyltransferase
- Alcohol Oxidoreductases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Genes, Bacterial
- Genetic Complementation Test
- Histidine
- Molecular Sequence Data
- Mutation