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Article

Cloning of mycobacterial histidine synthesis genes by complementation of a Mycobacterium smegmatis auxotroph.

Molecular microbiology - 1 Oct 1992

Hinshelwood S, Stoker N G

Abstract excerpt

Histidine-requiring auxotrophs of Mycobacterium smegmatis were isolated following N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of these mutants, his5, was transformed with an M. smegmatis shuttle cosmid library, and complementing clones were isolated at a frequency of approximately 1%. A 2.3 kb fragment was subcloned and sequenced, and found to contain the start of an operon including the hisD gene and...

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Source

Identifiers

PMID: 1435262
DOI: 10.1111/j.1365-2958.1992.tb01468.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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