Article
Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis.
Journal of bacteriology - 1 Nov 2005
Bhatt Apoorva, Kremer Laurent, Dai Annie Z, Sacchettini James C, Jacobs William R
Abstract excerpt
Inhibition or inactivation of InhA, a fatty acid synthase II (FASII) enzyme, leads to mycobacterial cell lysis. To determine whether inactivation of other enzymes of the mycolic acid-synthesizing FASII complex also leads to lysis, we characterized the essentiality of two beta-ketoacyl-acyl carrier protein synthases, KasA and KasB, in Mycobacterium smegmatis. Using specialized transduction for allelic exchange,...
Topics
- 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
- Bacterial Proteins
- Bacteriolysis
- Fatty Acid Synthases
- Genes, Bacterial
- Genes, Essential
- Microscopy, Electron, Scanning
- Mutation
- Mycobacterium smegmatis
- Mycolic Acids
- Oxidoreductases
- Transduction, Genetic