Article
amiA is a negative regulator of acetamidase expression in Mycobacterium smegmatis.
BMC microbiology - 1 Jan 2001
Parish T, Turner J, Stoker N G
Abstract excerpt
BACKGROUND: The acetamidase of Mycobacterium smegmatis is a highly inducible enzyme. Expression of this enzyme is increased 100-fold when the substrate acetamide is present. The acetamidase gene is found immediately downstream of three open reading frames. Two of these are proposed to be involved in regulation. RESULTS: We constructed a deletion mutant in one of the upstream ORFs (amiA). This mutant (Mad1) showed...
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