A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

doi:10.1128/jb.179.18.5827-5834.1997

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A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

Journal of bacteriology - 1 Sept 1997

Rappleye C A, Roth J R

Abstract excerpt

A new Tn10-based transposon has been constructed and used to isolate insertion mutations with tetracycline-conditional phenotypes. Classes of mutants include conditional lethal mutations, conditional auxotrophs, and conditional mutants of the eut (ethanolamine utilization) operon. The described mutations were made with a new derivative of Tn10dTet that we have called Tn10d(T-POP). Others have noted that...

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A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes.

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