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Article

Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.

Genetics - 1 Jul 2004

Hidalgo Alejandro A, Trombert A Nicole, Castro-Alonso J C, Santiviago Carlos A, Tesser Bruno R, Youderian Philip, Mora Guido C

Abstract excerpt

We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions upstream of essential genes. Generalized transducing phage P22 was used to introduce T-POP from a S. typhimurium donor into a S. typhi recipient. Chromosomal DNA was purified from the mutagenized donor strains, fragmented,...

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Identifiers

PMID: 15280224
DOI: 10.1534/genetics.104.026682
PMCID: PMC1470937

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Source receipt: pubmed, retrieved 7 Sept 2026.

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