Article
Preferential mutagenesis of lacZ integrated at unique sites in the Escherichia coli chromosome.
Molecular & general genetics : MGG - 1 Aug 1997
Liu S K, Tseng J N, Shiuan D, Hanawalt P C
Abstract excerpt
To study the variation in spontaneous mutation frequencies in different chromosomal domains, a mini-Mu-kan-lacZ- transposable element was constructed to insert the lacZ-(Trp570 --> Opal) allele into many different loci in the Escherichia coli chromosome. Papillation on MacConkey lactose plates was used to screen for mini-Mu insertion mutants with elevated levels of spontaneous mutagenesis of lacZop --> LacZ+;...
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