Article
Linker mutagenesis in the lacZ gene of Escherichia coli yields variants of active beta-galactosidase.
European journal of biochemistry - 1 Jan 1991
Breul A, Kuchinke W, von Wilcken-Bergmann B, Müller-Hill B
Abstract excerpt
Synthetic octameric oligonucleotides that code for a unique restriction site were cloned into a randomly linearized plasmid that carries the lacZ gene. The insertions were mapped by digestion with appropriate restriction endonucleases. 12 mutants were identified which carry an insertion within the lacZ gene and still express active beta-galactosidase. Small deletions or duplications of the wild-type sequence...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Deletion
- Chromosome Mapping
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Insertional
- Plasmids