Article
Detection and analysis of somatic mutations at a lacZ reporter locus in higher organisms: application to Mus musculus and Drosophila melanogaster.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Garcia Ana Maria, Busuttil Rita A, Rodriguez Armando, Cabrera Carlos, Lundell Martha, Dollé Martijn E T, Vijg Jan
Abstract excerpt
Methods to detect and analyze somatic mutations in higher organisms are critically important in view of their causal role in cancer, heritable diseases, and, possibly, aging. Here, we describe detailed protocols for the use of a mutational reporter system based on lacZ-containing plasmids integrated in the germline of Mus musculus and Drosophila melanogaster. Plasmids containing the bacterial lacZ gene integrated...
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