Article
Transfection of p53-knockout mouse fibroblasts with wild-type p53 increases the thermosensitivity and stimulates apoptosis induced by heat stress.
International journal of radiation oncology, biology, physics - 15 Jul 1997
Matsumoto H, Takahashi A, Wang X, Ohnishi K, Ohnishi T
Abstract excerpt
PURPOSE: The relationship between p53 functions and cellular thermosensitivity was evaluated using murine fibroblasts transfected with either wild-type p53 or mutated p53, or those with a null p53 genotype. METHODS AND MATERIALS: Cellular thermosensitivity was determined using the clonogenic assa...
Topics
- Animals
- Apoptosis
- Blotting, Western
- Cell Cycle
- Cell Survival
- Fibroblasts
- G1 Phase
- Genes, p53
- Genetic Vectors
- Genotype
- Hot Temperature
- Hyperthermia, Induced
- Mice
- Mice, Knockout
- Signal Transduction
- Stress, Physiological
- Transfection
- Tumor Suppressor Protein p53