Article
Isolation and characterization of peroxisome-deficient Chinese hamster ovary cell mutants representing human complementation group III.
Experimental cell research - 25 May 1997
Okumoto K, Bogaki A, Tateishi K, Tsukamoto T, Osumi T, Shimozawa N, Suzuki Y, Orii T, Fujiki Y
Abstract excerpt
We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent isolation of the Z65-type, PEX2-defective mutants. P9OH/UV-resistant cell colonies were examined...
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