Article
Isolation and characterization of peroxisome-deficient Chinese hamster ovary cell mutants representing human complementation group III.
Experimental cell research - 25 May 1997
Okumoto K, Bogaki A, Tateishi K, Tsukamoto T, Osumi T, Shimozawa N, Suzuki Y, Orii T, Fujiki Y
Abstract excerpt
We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were us...
Topics
- ATPases Associated with Diverse Cellular Activities
- Adenosine Triphosphatases
- Animals
- CHO Cells
- Cricetinae
- Drug Resistance
- Genetic Complementation Test
- Humans
- Lauric Acids
- Microbodies
- Mutation
- Peroxisomal Disorders
- Zellweger Syndrome