Article
A homogeneous method for genotyping with fluorescence polarization.
Clinical chemistry - 1 Aug 1997
Gibson N J, Gillard H L, Whitcombe D, Ferrie R M, Newton C R, Little S
Abstract excerpt
We combined the amplification refractory mutation system (ARMS) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein and the rhodamine dye 6-carboxyrhodamine were included in amplification mixes and were annealed to PCR products after...
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