Article
SNP genotyping with fluorescence polarization detection.
Human mutation - 1 Apr 2002
Kwok Pui-Yan
Abstract excerpt
When a fluorescent molecule is excited by plane polarized light, the fluorescence emitted is also polarized. The degree of fluorescence polarization (FP) detected, under constant temperature and solvent viscosity, is proportional to the molecular weight of the dye molecule. By monitoring the FP of a fluorescent dye, one can detect significant changes in the molecular weight of the molecule without separation or...
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