Article
Dimorphic primers derived from intron 1 for use in the molecular typing of HLA-B alleles.
Tissue antigens - 1 Jul 1997
Cereb N, Yang S Y
Abstract excerpt
We have identified a dimorphic site in intron 1 of the HLA-B gene. Oligotyping was performed on about 3000 samples using primers derived from this dimorphic site in combination with a locus-specific primer derived from intron 3. The distribution of B-alleles bearing each of the dimorphic sequences was approximately equal. These primers were mutually exclusive and yielded approximately 50% of the heterozygous...
Topics
- Alleles
- DNA Primers
- HLA-B Antigens
- Histocompatibility Testing
- Humans
- Introns
- Polymerase Chain Reaction