Directed evolution studies with combinatorial libraries of T4 lysozyme mutants.

doi:10.1007/BF01721324

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Article

Directed evolution studies with combinatorial libraries of T4 lysozyme mutants.

Molecular diversity - 1 Feb 1996

Patten P A, Sonoda T, Davis M M

Abstract excerpt

Gene duplication with divergence to new functions has been an important mechanism in protein evolution. However, the questions of how many new functions can arise from a particular ancestral gene and how many mutational steps are typically required to generate new functions have been difficult to...

Topics

Bacteriophage T4
Base Sequence
Binding Sites
Directed Molecular Evolution
Escherichia coli
Gene Library
Genes, Viral
Genetic Vectors
Molecular Sequence Data
Muramidase
Mutagenesis, Site-Directed
Mutation
Oligodeoxyribonucleotides

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