Removal of twelve C-terminal amino acids from firefly luciferase abolishes activity.

doi:10.1016/0006-291x(90)90697-l

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Article

Removal of twelve C-terminal amino acids from firefly luciferase abolishes activity.

Biochemical and biophysical research communications - 30 Oct 1990

Sala-Newby G, Kalsheker N, Campbell A K

Abstract excerpt

cDNA coding for the luciferase in the firefly Photinus pyralis was cloned using pcDV1 primer and Honjo linker containing SP6 RNA polymerase promoter. This enabled conditions to be established to produce mRNA, capped with m7 GpppG, in vitro and then translated to form light emitting protein. Full...

Topics

Animals
Base Sequence
Cloning, Molecular
Coleoptera
Genetic Variation
Luciferases
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction
Promoter Regions, Genetic
Protein Biosynthesis
Transcription, Genetic

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