Article
Characterization of translation initiation factor 5 (eIF5) from Saccharomyces cerevisiae. Functional homology with mammalian eIF5 and the effect of depletion of eIF5 on protein synthesis in vivo and in vitro.
The Journal of biological chemistry - 18 Jul 1997
Maiti T, Maitra U
Abstract excerpt
Eukaryotic translation initiation factor 5 (eIF5) interacts in vitro with the 40 S initiation complex (40 S.AUG.Met-tRNAf.eIF2.GTP) to mediate the hydrolysis of ribosome-bound GTP. In Saccharomyces cerevisiae, eIF5 is encoded by a single copy essential gene, TIF5, that encodes a protein of 45,346...
Topics
- Animals
- Cloning, Molecular
- Eukaryotic Initiation Factor-5
- Genes, Fungal
- Genotype
- Guanosine Triphosphate
- Kinetics
- Mammals
- Peptide Initiation Factors
- Polyribosomes
- Protein Biosynthesis
- RNA, Fungal
- RNA, Messenger
- Rats
- Recombinant Fusion Proteins
- Ribosomes
- Saccharomyces cerevisiae