Article
A single-tube multiplex system for the simultaneous detection of 10 common cystic fibrosis mutations.
Human mutation - 1 Jan 1995
Axton R A, Brock D J
Abstract excerpt
We describe a nonisotopic single-tube polymerase chain reaction (PCR) multiplex system that detects 10 of the more common cystic fibrosis (CF) mutations (delta F508, delta I507, V520F, G551D, G542X, R553X, R117H, 621 + 1G-->T, N1303K, A455E). The use of this method detects approximately 90% of th...
Topics
- Base Sequence
- Blood Proteins
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- Electrophoresis, Polyacrylamide Gel
- Female
- Genetic Testing
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Restriction Mapping
- United Kingdom
- White People