Identification of an essential cleavage site in ColE7 required for import and killing of cells.

doi:10.1074/jbc.M501216200

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Article

Identification of an essential cleavage site in ColE7 required for import and killing of cells.

The Journal of biological chemistry - 1 Jul 2005

Shi Zhonghao, Chak Kin-Fu, Yuan Hanna S

Abstract excerpt

Colicin E7 (ColE7), a nuclease toxin released from Escherichia coli, kills susceptible bacteria under environmental stress. Nuclease colicins are processed during translocation with only the cytotoxic nuclease domains traversing the inner membrane to cleave tRNA, rRNA, or DNA in the cytoplasm of target cells. In this study, we show that the E. coli periplasmic extract cleaves ColE7 between Lys(446) and Arg(447)...

Topics

Amino Acid Sequence
Arginine
Binding Sites
Blotting, Western
Circular Dichroism
Colicins
Cytoplasm
DNA
Deoxyribonucleases
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Endonucleases

Escherichia coli

Escherichia coli Proteins

Fluorescence Resonance Energy Transfer

Kinetics

Lysine

Models, Molecular

Source

Open in PubMed

Identifiers

PMID: 15857830
DOI: 10.1074/jbc.M501216200

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