Article
Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII.
Journal of microbiology (Seoul, Korea) - 1 Dec 2015
Jung Hyeim, Liang Junwei, Jung Yuna, Lim Dongbin
Abstract excerpt
Exonuclease VII (ExoVII) of Escherichia coli is a single strandspecific DNA nuclease composed of two different subunits: the large subunit, XseA, and the small subunit, XseB. In this study, we found that multicopy single-stranded DNAs (msDNAs), Ec83 and Ec78, are the in vivo substrates of ExoVII; the enzyme cuts the phosphodiester bond between the fourth and fifth nucleotides from the 5'end. We used this msDNA...
Topics
- Cloning, Molecular
- DNA Cleavage
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Escherichia coli Proteins
- Exodeoxyribonucleases
- Mutation
- Protein Subunits
- Proteolysis
- RNA, Bacterial