Article
C-terminal incorporation of fluorogenic and affinity labels using wild-type and mutagenized carboxypeptidase Y.
Analytical biochemistry - 15 May 1997
Stennicke H R, Olesen K, Sørensen S B, Breddam K
Abstract excerpt
The ability to carry out specific C-terminal modification or labeling of peptides and proteins has a broad range of applications. It is well established that this may be achieved by protease-catalyzed transacylation reactions and that carboxypeptidase Y (CPD-Y) is suitable for this due to its broad specificity and stability in the presence of denaturants. Furthermore, CPD-Y is characterized by a S'1 binding site...
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