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Article

Single-step purification of recombinant wild-type and mutant HIV-1 reverse transcriptase.

Protein expression and purification - 1 Feb 1996

Fletcher R S, Holleschak G, Nagy E, Arion D, Borkow G, Gu Z, Wainberg M A, Parniak M A

Abstract excerpt

We have devised a single-step method that enables purification of HIV-1 recombinant reverse transcriptase directly from bacterial lysates in less than 2 h. Clarified lysates are applied to commercial Q- and S-matrix cartridge columns connected in series. The columns are washed with low-salt buffer to remove unbound protein, then the Q column is removed and reverse transcriptase is eluted from the S column using a...

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Identifiers

PMID: 9172779
DOI: 10.1006/prep.1996.0004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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