Article
Insertion of a small peptide of six amino acids into the beta7-beta8 loop of the p51 subunit of HIV-1 reverse transcriptase perturbs the heterodimer and affects its activities.
BMC biochemistry - 18 Jun 2002
Pandey Pradeep K, Kaushik Neerja, Singh Kamalendra, Sharma Bechan, Upadhyay Alok K, Kumar Suriender, Harris Dylan, Pandey Virendra N
Abstract excerpt
BACKGROUND: HIV-1 RT is a heterodimeric enzyme, comprising of the p66 and p51 subunits. Earlier, we have shown that the beta7-beta8 loop of p51 is a key structural element for RT dimerization (Pandey et al., Biochemistry 40: 9505, 2001). Deletion or alanine substitution of four amino acid residues of this loop in the p51 subunit severely impaired DNA binding and catalytic activities of the enzyme. To further...
Topics
- Amino Acids
- DNA
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Dimerization
- HIV Reverse Transcriptase
- Models, Molecular
- Mutagenesis, Insertional
- Mutation
- Protein Binding