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Article

Formation of heterodimers of human-immunodeficiency-virus-type-1 reverse transcriptase by recombination of separately purified subunits.

European journal of biochemistry - 1 Jun 1992

Stammers D K, Ross C K, Idriss H, Lowe D M

Abstract excerpt

Human-immunodeficiency-virus-type-1 reverse transcriptase exists in virions as a heterodimer of a M(r) 66,000 subunit and its C-terminally truncated form of M(r) 51,000, but, when expressed as a recombinant M(r) 66,000 protein, a mixture of heterodimers and homodimers results which co-purify by most conventional techniques. We describe a method of hydrophobic chromatography which gives baseline separation of...

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PMID: 1375916
DOI: 10.1111/j.1432-1033.1992.tb16944.x

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