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Article

An integrated system to study multiply substituted human immunodeficiency virus type 1 reverse transcriptase.

Analytical biochemistry - 1 May 2001

Boretto J, Longhi S, Navarro J M, Selmi B, Sire J, Canard B

Abstract excerpt

We describe a gene system allowing the facile production of multiply substituted reverse transcriptases (RTs), the enzymatic characterization of these purified RTs, and the study of these mutations in the defined genetic background of the macrophagetropic, non-laboratory-adapted human immunodeficiency virus type 1 (HIV-1) AD8 strain. Thirteen unique silent restriction sites were introduced in the pol gene...

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Identifiers

PMID: 11319828
DOI: 10.1006/abio.2001.5045

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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