Article
Production and characterization of a novel monoclonal antibody against phosphorylated T cell receptor zeta chain.
Hybridoma - 1 Apr 1997
Conrad P J, Seng T, Barbosa J, Vasavada H, Blue M L
Abstract excerpt
One of the earliest events following T cell receptor (TCR) triggering is the activation of the protein kinase Lck and induction of tyrosine phosphorylation of zeta, the major signal transduction subunit of the T cell receptor complex. Here we report the generation and characterization of a monocl...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antibody Specificity
- Blotting, Western
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Flow Cytometry
- Humans
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Phosphoproteins
- Phosphorylation
- Precipitin Tests
- Receptors, Antigen, T-Cell
- Recombinant Proteins