Article
Isolation and characterization of Chinese hamster ovary cell mutants defective in assembly of peroxisomes.
The Journal of cell biology - 1 Mar 1990
Tsukamoto T, Yokota S, Fujiki Y
Abstract excerpt
We made use of autoradiographic screening to isolate two Chinese hamster ovary (CHO) cell mutants deficient in peroxisomal dihydroxyacetonephosphate acyltransferase, a key enzyme for the biosynthesis of ether glycerolipids such as plasmalogens. Morphological analysis revealed no evidence of peroxisome in these mutants. Catalase was as active as in the normal cells but was not sedimentable. Pulse-chase...
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