Article
Transcription Analysis of the Genes <i>tcdA‐E</i> of the Pathogenicity Locus of <i>Clostridium Difficile</i>
1 Mar 1997
Abstract excerpt
To analyse the transcription pattern of the five tcdA-E genes of the pathogenicity locus (PaLoc) of Clostridium difficile a protocol was established to purify RNA from strain VPI10463. Transcription analysis of the five tcdA-E genes showed that they were all transcribed. In the early exponential phase, a high level of tcdC and low levels of tcdA,B,D,E transcripts were detectable; this was inverted in the...
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