Article
Essential lysine residues in the N-terminal and the C-terminal domain of human adenylate kinase interact with adenine nucleotides as found by site-directed random mutagenesis.
Biochemistry - 1 Apr 1997
Ayabe T, Takenaka H, Takenaka O, Sumida M, Maruyama H, Onitsuka T, Shibata K, Uesugi S, Hamada M
Abstract excerpt
To elucidate the minimum requirement of amino acid residues for the active center in human adenylate kinase (hAK1), we carried out random site-directed mutagenesis of key lysine residues (K9, K21, K27, K31, K63, K131, and K194), which were conserved in mammalian AK1 species, with the pMEX8-hAK1 p...
Topics
- Adenine Nucleotides
- Adenylate Kinase
- Binding Sites
- DNA Primers
- Humans
- Kinetics
- Lysine
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Conformation