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Article

Site-directed random mutagenesis of AMP-binding residues in adenylate kinase.

Journal of biochemistry - 1 Nov 1993

Okajima T, Tanizawa K, Fukui T

Abstract excerpt

Two highly conservative residues, Val67 and Gln101, in adenylate kinase are located in the hydrophobic region putatively involved in binding of the adenine ring of AMP. We have performed polymerase chain reaction-based random mutagenesis of the two residues using recombinant chicken muscle adenylate kinase cDNA as a template. The synthetic oligonucleotide primers contained A,G,C,T-mixed bases in the codons...

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PMID: 8113212
DOI: 10.1093/oxfordjournals.jbchem.a124228

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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