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Article

In vitro mutagenesis studies at the arginine residues of adenylate kinase. A revised binding site for AMP in the X-ray-deduced model.

Biochemistry - 6 Feb 1990

Kim H J, Nishikawa S, Tokutomi Y, Takenaka H, Hamada M, Kuby S A, Uesugi S

Abstract excerpt

Although X-ray crystallographic and NMR studies have been made on the adenylate kinases, the substrate-binding sites are not unequivocally established. In an attempt to shed light on the binding sites for MgATP2- and for AMP2- in human cytosolic adenylate kinase (EC 2.7.4.3, hAK1), we have investigated the enzymic effects of replacement of the arginine residues (R44, R132, R138, and R149), which had been assumed...

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PMID: 2157484
DOI: 10.1021/bi00457a002

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