Article
In vitro mutagenesis studies at the arginine residues of adenylate kinase. A revised binding site for AMP in the X-ray-deduced model.
Biochemistry - 6 Feb 1990
Kim H J, Nishikawa S, Tokutomi Y, Takenaka H, Hamada M, Kuby S A, Uesugi S
Abstract excerpt
Although X-ray crystallographic and NMR studies have been made on the adenylate kinases, the substrate-binding sites are not unequivocally established. In an attempt to shed light on the binding sites for MgATP2- and for AMP2- in human cytosolic adenylate kinase (EC 2.7.4.3, hAK1), we have investigated the enzymic effects of replacement of the arginine residues (R44, R132, R138, and R149), which had been assumed...
Topics
- Adenosine Monophosphate
- Adenylate Kinase
- Arginine
- Base Sequence
- Binding Sites
- Cytosol
- Humans
- Kinetics
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data