Article
Characterization of a temperature-sensitive Escherichia coli mutant and revertants with altered seryl-tRNA synthetase activity.
Journal of bacteriology - 1 Apr 1997
Ferri M L, Vincent C, Leberman R, Härtlein M
Abstract excerpt
A mutation in the structural gene coding for seryl-tRNA synthetase in temperature-sensitive Escherichia coli K28 has been reported to alter the level of enzyme expression at high temperature (R. J. Hill and W. Konigsberg, J. Bacteriol. 141:1163-1169, 1980). We identified this mutation as a C-->T transition in the first base of codon 386, resulting in a replacement of histidine by tyrosine. The steady-state levels...
Topics
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Mutation
- RNA, Messenger
- Serine-tRNA Ligase
- Temperature