Article
Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid.
Molecular & general genetics : MGG - 1 Apr 1993
Masuda Y, Tsuchimoto S, Nishimura A, Ohtsubo E
Abstract excerpt
The pem locus, which is responsible for the stable maintenance of the low copy number plasmid R100, contains the pemK gene, whose product has been shown to be a growth inhibitor. Here, we attempted to isolate mutants which became tolerant to transient induction of the PemK protein. We obtained 20 mutants (here called pkt for PemK tolerance), of which 9 were temperature sensitive for growth. We analyzed the nine...
Topics
- Amino Acyl-tRNA Synthetases
- Aspartate-tRNA Ligase
- Bacterial Proteins
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genetic Complementation Test
- Genotype