Article
ABO genotyping by PCR-direct sequencing.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Feb 1997
Nata M, Kanetake J, Adachi N, Hashiyada M, Aoki Y, Sagisaka K
Abstract excerpt
The PCR-direct sequence method was applied to ABO genotyping. At the 261st nucleotide of the genes of A and B glycosyltrasferase, it was easily detected that the nucleotide was guanine in AA, AB and BB genotypes and that the nucleotide was ademine in only OO. In AO and BO, substitution of A to G was confirmed by the dye primer method, but it was difficult to detect correctly by the dye terminator method. At the...
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