Article
ABO genotyping by TaqMan assay and allele frequencies in a Japanese population.
Legal medicine (Tokyo, Japan) - 1 Mar 2013
Maeda Kazuho, Nakamura Shigeki, Murakami Chikako, Irie Wataru, Watanabe Toshimasa, Sasaki Chizuko, Nakamaru Naomi, Furukawa Masataka, Kurihara Katsuyoshi
Abstract excerpt
ABO genotyping have become common tools for forensic casework. We developed a new rapid ABO genotyping method using a fast real-time PCR system with the TaqMan® Sample-to-SNP™ Kit. Eight single nucleotide polymorphism (SNP) sites in the ABO gene (nt 261, 297, 467, 657, 703, 829, 930 and 1061) were selected to determine the ABO genotypes. ABO genotypes were easily determined by examining allelic discrimination...
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