Article
Determination of ABO genotypes by real-time PCR using allele-specific primers.
Legal medicine (Tokyo, Japan) - 1 Jan 2012
Muro Tomonori, Fujihara Junko, Imamura Shinji, Nakamura Hiroaki, Kimura-Kataoka Kaori, Toga Tomoko, Iida Reiko, Yasuda Toshihiro, Takeshita Haruo
Abstract excerpt
ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs)...
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