Article
ABO genotyping by PCR-RFLP and cloning and sequencing.
Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur - 1 Dec 2004
Haak Wolfgang, Burger Joachim, Alt Kurt Werner
Abstract excerpt
A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications. Four fragments of exon 6 and 7 of the ABO gene were amplified and digested by in total 7...
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