Article
ABO genotyping by capillary electrophoresis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Lee James Chun-I, Hsieh Hsing-Mei, Teng Hsiao-Feng, Tsai Li-Chin
Abstract excerpt
Single-strand conformation polymorphism by capillary electrophoresis (SSCP-CE) has been developed to detect single nucleotide mutations. This method is used to identify the ABO alleles A(1), A(1v), B, O(1), O(1v), and O(2) in this chapter. Four amplicons (112, 121, 123, and 160 bp) labeled with fluorescence are separately amplified by polymerase chain reaction from exons 6 and 7 of ABO gene. These four fragments...
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