Article
ABO genotyping following a single PCR amplification.
Journal of forensic sciences - 1 Mar 1996
Akane A, Yoshimura S, Yoshida M, Okii Y, Watabiki T, Matsubara K, Kimura K
Abstract excerpt
Using primers designed by Lee and Chang, 200 base-pair (bp) fragment of ABO locus was amplified by PCR, which spans the site of the single nucleotide deletion associated with O allele. O allele could be identified by Kpn I digestion of the PCR product as reported. A and B alleles were also distin...
Topics
- ABO Blood-Group System
- Alleles
- Base Sequence
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Restriction Mapping