Article
ABO genotyping by polymerase chain reaction.
Journal of forensic sciences - 1 Sept 1992
Lee J C, Chang J G
Abstract excerpt
ABO blood group system's genotyping by polymerase chain reaction in genomic DNA level is developed. The positions of nucleotide 258 and 700 of cDNA from A transferase were used to distinguish A, B, and O alleles by restriction enzyme digestion. To identify the 258th nucleotide, a 199- or 200-bp DNA fragment was amplified by PCR and digested with Kpn I. For the 700th nucleotide, a 128-bp PCR amplified fragment was...
Topics
- ABO Blood-Group System
- Base Sequence
- Blood Stains
- Bone and Bones
- DNA
- Female
- Forensic Medicine
- Gene Amplification
- Genotype
- Hair
- Humans
- Molecular Sequence Data
