Article
Rapid and clear detection of ABO genotypes by simultaneous PCR-RFLP method.
Journal of forensic sciences - 1 Nov 1996
Tun Z, Honda K, Nakatome M, Islam M N, Bai H, Ogura Y, Kuroki H, Yamazaki M, Terada M, Wakasugi C
Abstract excerpt
We reported a new approach of ABO genotyping by a polymerase chain reaction and restriction fragment length polymorphism method. Instead of amplifying the loci containing the positions of nucleotides 258 and 700 of cDNA of the A transferase separately, we successfully amplified these 2 loci together in one reaction mixture using 2 sets of primers. The amplified DNA products were digested at the same time with...
Topics
- ABO Blood-Group System
- DNA Fingerprinting
- Forensic Medicine
- Gene Amplification
- Genotype
- Humans
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length