Time-resolved spectroscopy of the early photolysis intermediates of rhodopsin Schiff base counterion mutants.

doi:10.1021/bi962320u

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Article

Time-resolved spectroscopy of the early photolysis intermediates of rhodopsin Schiff base counterion mutants.

Biochemistry - 25 Feb 1997

Jäger S, Lewis J W, Zvyaga T A, Szundi I, Sakmar T P, Kliger D S

Abstract excerpt

Time-resolved absorption difference spectra of COS-cell expressed rhodopsin and rhodopsin mutants (E113D, E113A/A117E, and G90D), solubilized in detergent, were collected from 20 ns to 510 ms after laser photolysis with 7 ns pulses (lambda(max) = 477 nm). The data were analyzed using a global exp...

Topics

Animals
COS Cells
Cattle
Models, Molecular
Mutagenesis, Site-Directed
Mutation
Photolysis
Rhodopsin
Spectrum Analysis, Raman

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